OUR RESEARCH PROGRAM covers all aspects of peptide chromatography for advanced application of analytical and proteomic peptide HPLC:

1. Continuous development of benchmark peptide reversed-phase HPLC prediction tool Sequence-Specific Retention Calculator (SSRCalc).
2. Standardization of peptide HPLC separations: retention standards, retention libraries.
3. Modeling reversed-phase HPLC for peptides carrying chemical and post-translational modifications, products for cross-linking proteomic studies.
4. Reversed-phase HPLC fundamentals: linear solvent strength theory; studying the effects of separation conditions.
5. Modeling alternative peptide separation modes: Hydrophilic Interaction Liquid Chromatography (HILIC), Cation and Anion Exchange separations (SCX, SAX), Capillary Zone Electrophoresis (CZE), Mixed-mode Separations, Ion Mobility Spectroscopy (IMS).
6. Development of advanced peptide multidimensional (2D and 3D) separation techniques, methods for evaluating peptide separation orthogonality and novel peptide fractionation protocols (P-SLICY - Proteome Selective Isolation Chromatography).

1. Continuous development of benchmark peptide reversed-phase HPLC prediction tool Sequence-Specific Retention Calculator (SSRCalc). Building on prior research in this area by Prof. R.S. Hodges lab, we introduced first sequence-specific peptide retention prediction model [] using just 349 peptides separated under C18 - 0.1% TFA conditions. Subsequently we went to study various sequence-specific features of peptide PRLC separations, focusing primarily on C18 - 0.1% formic acid conditions widely used for proteomic applications.

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2. Standardization of peptide HPLC separations: retention standards, retention libraries.

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