Biography
Born and raised in the tropical Nanaimo, BC, Darien start his life in research at Vancouver Island University (BSc) and University of Victoria (MSc). Following his interest in fundamental physical chemistry and prediction models - he joined the SSRCalc group to develop Proteome Selective Isolation Chromatography (P-SLICY) and developing in-silico techniques to enhance 2D chromatography in Proteomics.
Select Publications
Sequence-Specific Model for Predicting Peptide Collision Cross Section Values in Proteomic Ion Mobility Spectrometry
The contribution of peptide amino acid sequence to collision cross section values (CCS) has been investigated using a dataset of ∼134 000 peptides of four different charge states (1+ to 4+). The migration data were acquired using a two-dimensional liquid chromatography (LC)/trapped ion mobility spectrometry/quadrupole/time-of-flight mass spectrometry (MS) analysis of HeLa cell digests created using seven different proteases and was converted to CCS values.
pubs.acs.org
Separation Orthogonality in Liquid Chromatography-Mass Spectrometry for Proteomic Applications: Comparison of 16 Different Two-Dimensional Combinations
Peptide separation orthogonality for 16 different 2D LC-ESI MS systems has been evaluated. To compare and contrast the behavior of the first dimension columns, a large proteomic retention data set of ∼30 000 tryptic peptides was collected for each 2D pairing.
pubs.acs.org
